H-b index calculator is a web-based tool for designing efficient siRNA. By calculating H-b index for different regions of the target protein, the most efficient siRNA can be determined.

Please refer to the tutorial and our relevant publications:

Luo, K.Q. and Chang, D.C., The gene-silencing efficiency of siRNA is strongly dependent on the local structure of mRNA at the targeted region. Biochem Biophys Res Commun, 2004. 318(1): p. 303-10.

A manuscript for this web server is in preparation.